Oligo Synthesis : CEPs
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dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1914-02 | dSpacer CE Phosphoramidite | 0.25g | £300.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1914-90 | dSpacer CE Phosphoramidite | 100µmoles | £92.00£64.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
| dSpacer CE Phosphoramidite |
Catalog Number: 10-1914-xx
Description: dSpacer CE Phosphoramidite
| 5"-O-Dimethoxytrityl-1",2"-Dideoxyribose-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C35H45N2O6P | M.W.: 620.73 | F.W.: 180.10 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
SPACER MODIFIERS
The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers
SPACER MODIFIERS
The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers
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dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
Material Safety Data Sheet
Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS
Glen Report 16.1: Minor Base and Related Novel Phosphoramidites
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dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
(1) M. Salunkhe, T.F. Wu, and R.L. Letsinger, J. Amer. Chem. Soc., 1992, 114, 8768-8772.(2) N.G. Dolinnaya, M. Blumenfeld, I.N. Merenkova, T.S. Oretskaya, N.F. Krynetskaya, M.G. Ivanovskaya, M. Vasseur, and Z.A. Shabarova, Nucleic Acids Res., 1993, 21, 5403-5407.(3) M. Takeshita, C.N. Chang, F. Johnson, S. Will, and A.P. Grollman, J. Biol. Chem., 1987, 262, 10171-10179.(4) M.W. Kalnik, C.N. Chang, A.P. Grollman, and D.J. Patel, Biochemistry, 1988, 27, 924-931.(5) H. Urata and M. Akagi, Tetrahedron Lett., 1993, 34, 4015-4018.(6) C.J. Marasco, N.J. Angelino, B. Paul, and B.J. Dolnick, Tetrahedron Lett., 1994, 35, 3029-3032.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
dSpacer CE Phosphoramidite
| 5"-O-Dimethoxytrityl-1",2"-Dideoxyribose-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C35H45N2O6P | M.W.: 620.73 | F.W.: 180.10 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, see http://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
| Results in stable abasic sites in oligonucleotides.Often called abasic furan or ab furan |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS |
| Glen Report 16.1: Minor Base and Related Novel Phosphoramidites |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 4.03 | 121 | 72.6 | 45.38 | 33 | 24.2 | 6.05 |
| 10-1914-90 | 100µmoles | .062grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 6.01 | .067 | 113.8 | 71.13 | 51.73 | 7.11 | |
| 10-1914-90 | 100µmoles | .062grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 6.01 | .067 | 115.4 | 72.13 | 52.45 | ||
| 10-1914-90 | 100µmoles | .062grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
dSpacer CE Phosphoramidite
dSpacer CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 4.03 | 121 | 72.6 | 45.38 | 33 | 24.2 | 6.05 |
| 10-1914-90 | 100µmoles | .062grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 6.01 | .07 | 113.8 | 71.13 | 51.73 | 7.11 | |
| 10-1914-90 | 100µmoles | .062grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1914-02 | 0.25grams | .25grams | 6.01 | .07 | 115.4 | 72.13 | 52.45 | ||
| 10-1914-90 | 100µmoles | .062grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
