MW:110,000Daltons
CatalyticSubunit=80,000Daltons
RegulatorySubunit=30,000Daltons
Frozenin20mMimidazole,5mMβ-mercaptoethanol,1mMEDTA,1mMEGTA,30%glycerol,pH6.8
Storage:-70°Corlower
BuyNativeHumanCalpain-1(u-Calpain)CAPN1,HumanErythrocytedirectfromthemanufacturer.USDPrice$110.00/100ug.Samedayshipping.Bulkqty.available.
Calpain-1(μ-Calpain)isacalciumactivatedneutralcysteineprotease.ItisaheterodimerofaCatalytic80kDasubunitanda30kDasubunit,whichrequires50μMCa2+forhalf-maximalactivityinvitro.Itsparticipationineventssuchascelldivision,signaltransduction,andlongtermpotentiationhasbeensuggested.Calpainshavealsobeenproposedtoplayaroleinvariouspathologicalprocessesassociatedwithalteredmetabolismandalteredcalciumhomeostasis.
Ref:Vilei,E.E.etal.1997.JournalofBIOLOGicalChemistry.272.41
Activity:Greaterthanorequalto1,000unitspermgprotein.
OneunitisdefinedastheamountofenzymethatwillhydrolyzeoneρmolSuc-LLVY-AMCperminuteat25°CusingtheCalpainActivityAssayKit,Fluorogenic(EMDCat.No.QIA120)
Purity:Greaterthanorequalto95%bySDS-PAGE.
Preparedfromwholebloodshowntobenon-reactiveforHBsAg,anti-HCV,anti-HBc,andnegativeforanti-HIV1&2byFDAapprovedtests.
AthensResearch&TechnologyproductsarelaboratoryreagentsandarenottobeadmiNISTeredtohumansorusedforanydrugpurpose.Forresearchorfurthermanufacturinguseonly.