Oligo Synthesis : CEPs
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2-Amino-dA-CE Phosphoramidite
2-Amino-dA-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1085-02 | 2-Amino-dA-CE Phosphoramidite | 0.25g | £200.00£190.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1085-90 | 2-Amino-dA-CE Phosphoramidite | 100µmoles | £100.00£95.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1085-95 | 2-Amino-dA-CE Phosphoramidite | 50µmoles | £56.00£53.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2-Amino-dA-CE Phosphoramidite
2-Amino-dA-CE Phosphoramidite
Glen Research
| 2-Amino-dA-CE Phosphoramidite |
Catalog Number: 10-1085-xx
Description: 2-Amino-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2,N6-bis(diisobutylaminomethylidene)-2,6-diamino-2"-deoxypurine riboside-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C58H83N10O6P | M.W.: 1047.33 | F.W.: 328.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling recommended.SEE TECHNICAL BULLETIN |
| Deprotection: See Technical Bulletin for details (www.glenresearch.com/Technical/TB_2-Amino-dA.pdf). Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
bases affecting duplex stability
C-5 methyl pyrimidine nucleosides are known to stabilize duplexes relative to the non-methylated bases. Therefore, enhanced binding can be achieved using 5-methyl-dC in place of dC, duplex melting temperature being increased by 1.3°. Improved stacking in this case is believed to be brought about by elimination of water molecules from the duplex. 2,6-Diaminopurine 2"-deoxyriboside (2-amino-dA) forms an additional hydrogen bond with Thymidine, thereby leading to duplex stabilization with a melting temperature increase of 3°. Our 2-amino-dA monomer exhibits fast and effective deprotection in ammonium hydroxide and it is stabilized to depurination during synthesis. Sequences with high GC content may contain mismatches and still hybridize because of the high stability of the G-C base pair. The N4-ethyl analogue of dC (N4-Et-dC) hybridizes specifically to natural dG but the stability of the base pair is reduced to about the level of an AT base pair.AP-dC (G-clamp) enhances oligo hybridization since the AP-C....G base pair contains 4 hydrogen bonds, which makes the interaction much stronger than the regular C....G base pair with its 3 hydrogen bonds.
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2-Amino-dA-CE Phosphoramidite
2-Amino-dA-CE Phosphoramidite
Glen Research
MSDS
Glen Report 11.1
Glen Report 23.2
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2-Amino-dA-CE Phosphoramidite
2-Amino-dA-CE Phosphoramidite
Glen Research
2-Amino-dA-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2,N6-bis(diisobutylaminomethylidene)-2,6-diamino-2"-deoxypurine riboside-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C58H83N10O6P | M.W.: 1047.33 | F.W.: 328.22 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 15 minute coupling recommended.SEE TECHNICAL BULLETIN |
| Deprotection: SEE TECHNICAL BULLETIN (http://www.glenresearch.com/Technical/TB_2-Amino-dA.pdf) Technical Bulletin |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 2-3 days |
| The convertable nucleoside 10-1071 S6-DNP-dG-CE Phosphoramidite can also be used to incorporate 2-Amino-dA by post synthesis modification of the base after oligonucleotide synthesis. 2-Amino-dA-CED Phosphoramidite has proven to be a more reliable reagent for incorporation of 2-Amino-dA into an oligonucleotide.Deprotection:Acetyl-protected dC must be used during synthesis if the AMA deprotection is to be used or transamination will occur. If benzoyl-dC is used during synthesis ammonium Hydroxide for 7 days at 55oC may be used for deprotection. |
| Material Safety Data Sheet |
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1085 | 2,6-diaminoPurine | 278 | 10.2 | 255 | 9.3 | 8.5 |
Literature Highlights
| Glen Report 11.1: USING MODIFIED BASES TO OPTIMIZE HYBRIDIZATION |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 2.39 | 66.33 | 39.8 | 24.88 | 18.09 | 13.27 | 3.32 |
| 10-1085-90 | 100µmoles | .105grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1085-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .067 | 64.8 | 40.5 | 29.45 | 4.05 | |
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1085-95 | 50µmoles | .052grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .067 | 66.4 | 41.5 | 30.18 | ||
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1085-95 | 50µmoles | .052grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2-Amino-dA-CE Phosphoramidite
2-Amino-dA-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1085 | 2,6-diaminoPurine | 278 | 10.2 | 255 | 9.3 | 8.5 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 2.39 | 66.33 | 39.8 | 24.88 | 18.09 | 13.27 | 3.32 |
| 10-1085-90 | 100µmoles | .105grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1085-95 | 50µmoles | .052grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .07 | 64.8 | 40.5 | 29.45 | 4.05 | |
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1085-95 | 50µmoles | .052grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1085-02 | 0.25grams | .25grams | 3.56 | .07 | 66.4 | 41.5 | 30.18 | ||
| 10-1085-90 | 100µmoles | .105grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1085-95 | 50µmoles | .052grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
