Oligo Synthesis : CEPs
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Azobenzene Phosphoramidite
Azobenzene Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-5800-02 | Azobenzene Phosphoramidite | 0.25g | £440.00£418.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5800-90 | Azobenzene Phosphoramidite | 100µmoles | £160.00£152.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-5800-95 | Azobenzene Phosphoramidite | 50µmoles | £84.00£79.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Azobenzene Phosphoramidite
Azobenzene Phosphoramidite
Glen Research
| Azobenzene Phosphoramidite |
Catalog Number: 10-5800-xx
Description: Azobenzene Phosphoramidite
| 3-O-(Dimethoxytrityl)-2-N-(4-carboxyazobenzene)-D-threonin-1-yl-O-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C47H54N5O6P | M.W.: 815.94 | F.W.: 375.32 |
Diluent: Anhydrous Acetonitrile |
| Coupling:10 minutes |
| Deprotection:As required by nucleobases.Compatible with Ammonium Hydroxide for 17 hours at roomtemperature, Ammonium hydroxide/40% methylamine 1:1 (AMA) for 10 minutes at 65 °C or UltraMild deprotection conditions. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 1-2 days |
Labelling for Photo-Regulation ofOligonucleotides
Photo-control, the use of ultraviolet or visible light to control a reaction,has a number of advantages over other external stimuli:
- Light does not introduce contaminants into the reactionsystem,
- Excitation wavelength can be controlled through the design ofthe photo-responsive molecule
- It is now straightforward to control irradiation time and/orlocal excitation.
When a photo-responsive molecule is directly attached to DNA as a receptor,photo-regulation of the bioprocess regulated by that DNA molecule could, inprinciple, be achieved. Such photo-responsive DNA could also be used as a switchin a DNA-based nano-machine. Professor Hiroyuki Asanuma and his group at thedepartment of Molecular Design and Engineering of the Graduate School ofEngineering of the Nagoya University (Japan) have developed an efficient methodto achieve this goal. They have attached azobenzene to DNA and made itphoto-responsive1,2. Azobenzene is a typical photo-responsivemolecule that isomerizes from its planar trans-form to the non-planar cis-formafter UV-light irradiation with a wavelength between 300 nm and 400 nm (lmax isaround 330 nm). Interestingly, the system reverts from the cis-form to thetrans-form after further irradiation with visible light (wavelength over 400nm). This process is completely reversible, and the azobenzene group does notdecompose or induce undesirable side reactions even on repeated trans-cisisomerization. By introducing azobenzenes into DNA through D-threoninol as alinker, Asanuma and co-workers succeeded in achieving photo-regulation of:
- Formation and dissociation of a DNA duplex3,4 and
- Transcription by T7-RNA polymerasereaction5,6,7.
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Azobenzene Phosphoramidite
Azobenzene Phosphoramidite
Glen Research
MSDS
Glen Report 21.2: New Product - Azobenzene phosphoramidite for the introduction of photo-regulated functions in DNA
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Azobenzene Phosphoramidite
Azobenzene Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-5800-95 | 50µmoles | .041grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| 10-5800-90 | 100µmoles | .082grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-5800-02 | 0.25grams | .25grams | 3.06 | 88.67 | 53.2 | 33.25 | 24.18 | 17.73 | 4.43 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-5800-95 | 50µmoles | .041grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| 10-5800-90 | 100µmoles | .082grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-5800-02 | 0.25grams | .25grams | 4.57 | .07 | 85 | 53.13 | 38.64 | 5.31 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-5800-95 | 50µmoles | .041grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
| 10-5800-90 | 100µmoles | .082grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-5800-02 | 0.25grams | .25grams | 4.57 | .07 | 86.6 | 54.13 | 39.36 | ||
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