Oligo Synthesis : CEPs
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8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1028-02 | 8-oxo-dG-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1028-90 | 8-oxo-dG-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1028-95 | 8-oxo-dG-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
| 8-oxo-dG-CE Phosphoramidite |
Catalog Number: 10-1028-xx
Description: 8-oxo-dG-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-isobutyryl-8-oxo-deoxyGuanosine-3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C44H54N7O9P | M.W.: 855.93 | F.W.: 345.21 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 24 hours DNA Damage/RepairCellular DNA is constantly being damaged by oxidation andalkylation, by free radicals, and by ultraviolet and ionizing radiation. Thebody has therefore evolved a number of repair enzyme systems to excise andrepair these lesions. The 8-oxo purine monomers allow investigation of thestructure and activity of oligonucleotides containing an 8-oxo mutation which isformed naturally when DNA is subjected to oxidative conditions or ionizingradiation. 5,6-Dihydro pyrimidines are naturally occurring compounds that arestructural components of alanine transfer RNA. Dihydrouracil and the hydroxypyrimidines are major base damage products formed by exposure of DNA to ionizingradiation. |
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8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
MSDS
Glen Report 7.1: NEW MINOR BASES
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8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
Synthetic oligonucleotides containing 8-oxo-dG must be cleaved and deprotected with ammonium hydroxide containing 0.25M 2-mercaptoethanol to avoid oxidative degradation of 8-oxo-dG sites.
Frequently Asked Technical Question
QUESTION: How do you deprotect 8-oxo-dG?
RESPONSE:Cleave and deprotect with ammonium hydroxide containing 0.25M 2-mercaptoethanol 17 hours at 55°C to avoid oxidative degradation of the 8-oxo-dG site
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
8-oxo-dG-CE Phosphoramidite
8-oxo-dG-CE Phosphoramidite
Glen Research
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1028 | 8-oxo-dG | 294 | 5.2 | 250 | 6.7 | 5.9 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 2.92 | 84 | 50.4 | 31.5 | 22.91 | 16.8 | 4.2 |
| 10-1028-90 | 100µmoles | .086grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1028-95 | 50µmoles | .043grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .07 | 80.8 | 50.5 | 36.73 | 5.05 | |
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1028-95 | 50µmoles | .043grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1028-02 | 0.25grams | .25grams | 4.36 | .07 | 82.4 | 51.5 | 37.45 | ||
| 10-1028-90 | 100µmoles | .086grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1028-95 | 50µmoles | .043grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
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