Oligo Synthesis : CEPs
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6-thio-dG-CE Phosphoramidite
6-thio-dG-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1072-02 | 6-thio-dG-CE Phosphoramidite | 0.25g | £780.00£741.00Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1072-90 | 6-thio-dG-CE Phosphoramidite | 100µmoles | £284.00£269.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1072-95 | 6-thio-dG-CE Phosphoramidite | 50µmoles | £142.00£134.90Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
6-thio-dG-CE Phosphoramidite
6-thio-dG-CE Phosphoramidite
Glen Research
Catalog Number: 10-1072-xx
Description: 6-thio-dG-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-trifluoroacetyl-2"-deoxy-6-(2-cyanoethyl)thio-Guanosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H50F3N8O7PS | M.W.: 934.97 | F.W.: 345.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time.Use 0.02 M Iodine for Oxidation. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 5hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
Stability in Solution: 24 hours The C-nucleoside2’-deoxypseudouridine, in contrast to dU, forms stable C:pseudoU-A triplets.2-Aminopurine lacks groups critical for hydrogen bonding and is a mildlyfluorescent base. Demand for sulfur modified bases continues to expand forinvestigations of oligonucleotide structure, but primarily for cross-linkingpurposes. 6-Thio-dG, 4-Thio-dT and 4-thio-dU are very useful modifications forphoto cross-linking and photoaffinity labelling experiments. Oligos containing2-thio-dT are useful in examining protein-DNA interaction by acting asphotosensitizing probes. The thiocarbonyl group in 2-thio-dT is especiallyinteresting in that it is available to react with compounds associating with theminor groove of DNA. 2-Amino-A forms a very stable base pair with T containingthree hydrogen bonds but the stability of the base pair with 2-thio-T is greatlydiminished. Due to steric interactions between the 2-thio group of thymidine andthe 2-amino group of 2-amino-A, the base pair contains only a single hydrogenbond. Oligos containing 2-amino-dA and 2-thio-dT exhibit high affinity fornatural oligonucleotides but show little affinity for other similar oligos evenof a complementary sequence. |
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
6-thio-dG-CE Phosphoramidite
6-thio-dG-CE Phosphoramidite
Glen Research
MSDS
Glen Report 11.1: MORE NOVEL MONOMERS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
6-thio-dG-CE Phosphoramidite
6-thio-dG-CE Phosphoramidite
Glen Research
6-thio-dG-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-trifluoroacetyl-2"-deoxy-6-(2-cyanoethyl)thio-Guanosine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C45H50F3N8O7PS | M.W.: 934.97 | F.W.: 345.26 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Standard coupling time. Use 0.02 M Iodine for Oxidation. |
| Deprotection: Deprotect with1.0M DBU in anhydrous acetonitrile at Room Temperature for 5hrs to remove the cyanoethyl protection. Complete the deprotection with 50mM NaSH in concentrated NH4OH at Room Temperature for 24hrs. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
| Material Safety Data Sheet |
Literature Highlights
| Glen Report 11.1: MORE NOVEL MONOMERS |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 2.67 | 75.67 | 45.4 | 28.38 | 20.64 | 15.13 | 3.78 |
| 10-1072-90 | 100µmoles | .093grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1072-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .067 | 73.4 | 45.88 | 33.36 | 4.59 | |
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .067 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1072-95 | 50µmoles | .047grams | .75 | .067 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .067 | 75 | 46.88 | 34.09 | ||
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .067 | 25.2 | 15.75 | 11.45 | ||
| 10-1072-95 | 50µmoles | .047grams | .75 | .067 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
6-thio-dG-CE Phosphoramidite
6-thio-dG-CE Phosphoramidite
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link for more detailed usage information with the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 2.67 | 75.67 | 45.4 | 28.38 | 20.64 | 15.13 | 3.78 |
| 10-1072-90 | 100µmoles | .093grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| 10-1072-95 | 50µmoles | .047grams | .5 | 3.33 | 2 | 1.25 | .91 | .67 | .17 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .07 | 73.4 | 45.88 | 33.36 | 4.59 | |
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| 10-1072-95 | 50µmoles | .047grams | .75 | .07 | 8.6 | 5.38 | 3.91 | .54 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1072-02 | 0.25grams | .25grams | 3.99 | .07 | 75 | 46.88 | 34.09 | ||
| 10-1072-90 | 100µmoles | .093grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
| 10-1072-95 | 50µmoles | .047grams | .75 | .07 | 10.2 | 6.38 | 4.64 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
