Oligo Synthesis : CEPs
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5"-I-dT-CE Phosphoramidite
5"-I-dT-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1931-02 | 5"-I-dT-CE Phosphoramidite | 0.25g | £236.00£224.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1931-90 | 5"-I-dT-CE Phosphoramidite | 100µmoles | £68.00£64.60Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
5"-I-dT-CE Phosphoramidite
5"-I-dT-CE Phosphoramidite
Glen Research
| 5"-I-dT-CE Phosphoramidite |
Catalog Number: 10-1931-xx
Description: 5"-I-dT-CE Phosphoramidite
| 5"-Iodo-2"-deoxyThymidine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C19H30N4O5PI | M.W.: 552.35 | F.W.: 414.09 |
Diluent: Anhydrous Acetonitrile |
| Coupling: Monomers that allow for UltraMILD deprotection must be used. (Catalog Numbers: dA: 10-1601-xx, dC: 10-1015-xx, dG: 10-1621-xx, dT: 10-1030-xx. |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4hr at Room Temperature. (Deprotection may be carried out at room temperature in ammonium hydroxide for 24hr, however ~10% loss in iodine label will occur.) |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
Oligonucleotides prepared using 5’-Hexynyl Phosphoramidite are stable tostandard deprotection conditions and exhibit a slightly increased retention timeon RP HPLC. Azides are not compatible with oligonucleotide synthesis usingphosphoramidites so a post-synthesis reaction is required. Azidobutyrate NHSEster is used1 for azido-modification of amines at either the 3’-endor the 5’-end of an oligo and it can even be used for internal modification onan Amino-Modifier-C6 dX residue within the sequence. Specific to the5’-terminus, 5’-Bromohexyl Phosphoramidite is added in the last cycle. Thismodifier can then be easily transformed into a 5’-azido group by displacement ofbromide using sodium azide.2 Alkyne NHS ester allows thefunctionalization of an amino moiety in a variety of molecules, including DNAand RNA oligonucleotides as well as peptides or proteins. We also offer asynthesis support for labelling the 3’ terminus of oligonucleotides with analkyne group for use in Click Chemistry. This builds upon our 1,3-diol productportfolio with the serinol backbone. A 5’-iodo-modified oligonucleotide(prepared using 5’-Iodo-dT) can be quantitatively converted to the corresponding5’-azide.
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5"-I-dT-CE Phosphoramidite
5"-I-dT-CE Phosphoramidite
Glen Research
MSDS
Glen Report 10.1: NON-ENZYMATIC LIGATION OF SINGLE-STRANDED AND DUPLEX DNA
Glen Report 19.1: 5"-Hexynyl Phosphoramidite - Conjugation with a Click
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
5"-I-dT-CE Phosphoramidite
5"-I-dT-CE Phosphoramidite
Glen Research
| Conjugation with a Click |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1931-02 | 0.25grams | .25grams | 4.53 | 137.67 | 82.6 | 51.63 | 37.55 | 27.53 | 6.88 |
| 10-1931-90 | 100µmoles | .055grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1931-02 | 0.25grams | .25grams | 6.76 | .07 | 128.8 | 80.5 | 58.55 | 8.05 | |
| 10-1931-90 | 100µmoles | .055grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1931-02 | 0.25grams | .25grams | 6.76 | .07 | 130.4 | 81.5 | 59.27 | ||
| 10-1931-90 | 100µmoles | .055grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
