Oligo Synthesis : CEPs
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2"-OMe-iPr-Pac-G-CE Phosphoramidite
2"-OMe-iPr-Pac-G-CE Phosphoramidite
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-3621-02 | 2"-OMe-iPr-Pac-G-CE Phosphoramidite | 0.25g | £50.00£47.50Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3621-05 | 2"-OMe-iPr-Pac-G-CE Phosphoramidite | 0.5g | £100.00£95.00Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3621-10 | 2"-OMe-iPr-Pac-G-CE Phosphoramidite | 1.0g | £200.00£190.00Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-3621-90 | 2"-OMe-iPr-Pac-G-CE Phosphoramidite | 100µmoles | £30.00£28.50Offer until : 31-Dec-2020Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-OMe-iPr-Pac-G-CE Phosphoramidite
2"-OMe-iPr-Pac-G-CE Phosphoramidite
Glen Research
| 2"-OMe-iPr-Pac-G-CE Phosphoramidite |
Catalog Number: 10-3621-xx
Description: 2"-OMe-iPr-Pac-G-CE Phosphoramidite
| 5"-Dimethoxytrityl-N2-isopropylphenoxyacetyl-Guanosine,2"-O-methyl,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C52H62N7O10P | M.W.: 976.07 | F.W.: 359.24 |
Diluent: Anhydrous Acetonitrile |
| Coupling: 6 minute coupling time.To avoid any exchange of the iPr-Pac group on the dG with acetyl, use the UltraMild Cap Mix A (40-4210-xx/ 40-4212-xx). |
| Deprotection: UltraMILD deprotection: 0.05M Potassium Carbonate in Methanol, 4 hours at Room Temperature OR 2 hours at Room Temperature in 30% Ammonium Hydroxide. Technical Bulletin |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
UltraMild 2’-OMe-RNA
The use of UltraMild monomers in oligonucleotide synthesis has allowed verysensitive dyes like TAMRA, HEX and Cy5 to be used virtually routinely. The DNAand RNA monomers are currently available and we also provide this set of2’-OMe-RNA monomers. In our version of this chemistry, we use as protectinggroups phenoxyacetyl (Pac) for A, acetyl (Ac) for C, and isopropyl-phenoxyacetyl(iPr-Pac) for G.
It has become clear that acetic anhydride in the conventional capping mix cancause transamidation in situations where an amine protecting group is quitelabile. This leads to acetyl protection on the amino group that may be slow tobe removed. Consequently, if many dG residues are included in theoligonucleotide, we recommend the use of phenoxyacetic anhydride (Pac2O) in CapA. This modification removes the possibility of exchange of the iPr-Pacprotecting group on the dG with acetate from the acetic anhydride capping mix
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-iPr-Pac-G-CE Phosphoramidite
2"-OMe-iPr-Pac-G-CE Phosphoramidite
Glen Research
MSDS
Glen Report 15.1
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-OMe-iPr-Pac-G-CE Phosphoramidite
2"-OMe-iPr-Pac-G-CE Phosphoramidite
Glen Research
| BASES |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-3621-02 | 0.25grams | .25grams | 2.56 | 72 | 43.2 | 27 | 19.64 | 14.4 | 3.6 |
| 10-3621-05 | 0.5grams | .5grams | 5.12 | 157.33 | 94.4 | 59 | 42.91 | 31.47 | 7.87 |
| 10-3621-10 | 1.0gram | 1grams | 10.25 | 328.33 | 197 | 123.13 | 89.55 | 65.67 | 16.42 |
| 10-3621-90 | 100µmoles | .098grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-3621-02 | 0.25grams | .25grams | 3.82 | .07 | 70 | 43.75 | 31.82 | 4.38 | |
| 10-3621-05 | 0.5grams | .5grams | 7.65 | .07 | 146.6 | 91.63 | 66.64 | 9.16 | |
| 10-3621-10 | 1.0gram | 1grams | 15.29 | .07 | 299.4 | 187.13 | 136.09 | 18.71 | |
| 10-3621-90 | 100µmoles | .098grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-3621-02 | 0.25grams | .25grams | 3.82 | .07 | 71.6 | 44.75 | 32.55 | ||
| 10-3621-05 | 0.5grams | .5grams | 7.65 | .07 | 148.2 | 92.63 | 67.36 | ||
| 10-3621-10 | 1.0gram | 1grams | 15.29 | .07 | 301 | 188.13 | 136.82 | ||
| 10-3621-90 | 100µmoles | .098grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
