Oligo Synthesis : CEPs
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2"-DeoxyNebularine-CE Phosphoramidite (Purine)
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1041-02 | 2"-DeoxyNebularine-CE Phosphoramidite (Purine) | 0.25g | £204.00£193.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1041-90 | 2"-DeoxyNebularine-CE Phosphoramidite (Purine) | 100µmoles | £84.00£79.80Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Related products
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
Glen Research
| 2"-DeoxyNebularine-CE Phosphoramidite (Purine) |
Catalog Number: 10-1041-xx
Description: 2"-DeoxyNebularine-CE Phosphoramidite (Purine)
| 5"-Dimethoxytrityl-2"-deoxyNebularine,3"-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C40H47N6O6P | M.W.: 738.82 | F.W.: 298.19 |
Diluent: Anhydrous Acetonitrile |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Refrigerated storage, maximum of 2-8°C, dry |
| Stability in Solution: 24 hours |
duplex effects
The design of primers is frequently complicated by the degeneracy of the genetic code. Three strategies are now available to confront this problem. In the first, a mixed base addition (N) is used to form the degenerate site. This approach is best if the number of degenerate sites is small. A second option is the use of 2’-deoxyInosine or 2’-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other four bases. The third option is the use of a universal nucleoside. In this strategy, the base analog does not hybridize significantly to the other four bases and makes up some of the duplex destabilization by acting as an intercalating agent. 3-Nitropyrrole 2’-deoxynucleoside (M) is the first example of a set of universal bases. Subsequently, 5-nitroindole was determined to be an effective universal base and to be superior to 3-nitropyrrole, based on duplex melting experiments.
The modified bases designated P and K show considerable promise as degenerate bases. The pyrimidine derivative P, when introduced into oligonucleotides, base pairs with either A or G, while the purine derivative K base pairs with either C or T. A dP dK mix also can serve as a mixed base with much less degeneracy than dA dC dG dT (N).
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2"-DeoxyNebularine-CE Phosphoramidite (Purine)
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
Glen Research
MSDS
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
2"-DeoxyNebularine-CE Phosphoramidite (Purine)
Glen Research
The design of primers is frequently complicated by the degeneracy of the genetic code. Three strategies are now available to confront this problem.
- A mixed base addition (N) is used to form the degenerate site. This approach is best if the number of degenerate sites is small.
- The use of 2"-deoxyInosine or 2"-deoxyNebularine which exhibit low, but unequal, hydrogen bonding to the other 4 bases.
- The use of a universal nucleotide. In this strategy, the base analog does not hybridise significantly to the other 4 bases and makes up some of the duplex destabilisation by acting as an intercalating agent.
EXTINCTION DATA
| Item | Nucleoside | λMax-1 | Emax-1 | λMax-2 | Emax-2 | E260 |
| (nm) | (ml/µmole) | nm | (ml/µmole) | (ml/µmole) | ||
| 10-1041 | dNebularine | 262 1 | 7.1 | 7 |
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1041-02 | 0.25grams | .25grams | 3.38 | 99.33 | 59.6 | 37.25 | 27.09 | 19.87 | 4.97 |
| 10-1041-90 | 100µmoles | .074grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1041-02 | 0.25grams | .25grams | 5.05 | .07 | 94.6 | 59.13 | 43 | 5.91 | |
| 10-1041-90 | 100µmoles | .074grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1041-02 | 0.25grams | .25grams | 5.05 | .07 | 96.2 | 60.13 | 43.73 | ||
| 10-1041-90 | 100µmoles | .074grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
