Gel Electrophoresis: Gel Electrophoresis

Cambio high quality human placental genomic sonicated DNA (Hybridime)

DNA derived from placentae is composed of that from the foetal cells and also a small number of maternal cells. The maternal cells fall away with the placenta when the placenta is delivered following birth. Human placental genomic DNA is uniquely suited to many different uses in molecular biology. It can be used in applications where nucleic acid blocking is required such as Southern hybridisation, specialised cytogenetic assays, fluorescence in situ hybridisation (FISH), Northern hybridisation etc.

Cambio’s human placental genomic DNA is total genomic DNA and will specifically block human random repeat sequences making it an ideal blocker for hybridisation experiments.

Our Sonicated DNA can be supplied as convenient small pack sizes (10 mg) or in bulk (gram quantities) as required.

Applications

Blocking agent for a wide range of different DNA- or RNA-based applications, including but not limited to:

• Southern hybridization
• Northern hybridization
• Applications employing nucleic acid probes
• Nucleic acid binding assays
• Fluorescence in-situ Hybiridsation (FISH)
• Cytogenetic assays
• PCR standards
• As internal Controls

Please contact support@cambio.co.uk if you have specific requirements for your workflow for the peak size of sonicated DNA fragments.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

Weterman M, A, J, Wilbrink M, Janssen I, Janssen H, A, P, van den Berg E, Fisher S, E, Craig I, Geurts van Kessel A: Molecular cloning of the papillary renal cell carcinoma-associated translocation (X;1)(p11;q21) breakpoint. Cytogenet Genome Res 1996;75:2-6.Marcon, M., Briani, C., Ermani, M. et al. Positive correlation of CTG expansion and pharyngoesophageal alterations in myotonic dystrophy patientsItal J Neuro Sci (1998) 19: 75.Marian AJ Weterman, Fred van Ruissen, et al Copy number variation upstream of PMP22 in Charcot Marie Tooth disease, European Journal of Human Genetics volume 18, pages 421–428 (2010). Martienvan Asseldonk MargaSchepens et al, Construction of a 350-kb Sequence-Ready 11q13 Cosmid Contig Encompassing the Markers D11S4933 and D11S546: Mapping of 11 Genes and 3 Tumor-Associated Translocation Breakpoints, Genomics Volume 66, Issue 1, 15 May 2000, Pages 35-42.M. Gennarelli G. Novelli F. et al , Prediction of myotonic dystrophy clinical severity based on the number of intragenic [CTG]n trinucleotide repeats, AJMG, Volume 65, Issue4 11 November 1996 Pages 342-347Sharon Brookes, G. Alistair Lammie et al, Amplified region of chromosome band 11q13 in breast and squamous cell carcinomas encompasses three CpG islands telomeric of FGF3, including the expressed gene EMS1, Genes, Chromosomes & Cancer, Volume 6, Issue 4 April 1993 Pages 222-231.Massimo Gennarelli , Marco Lucarelli et al, Genomic instability associated with myotonic dystrophy does not involve p53 expression and activity, Cell biochemistry and function , Volume 16, Issue2 June 1998 Pages 117-122.Paola Melacini, Carla Villanova et al , Correlation between cardiac involvement and CTG trinucleotide repeat length in myotonic dystrophy, Journal of the American College of Cardiology Volume 25, Issue 1, January 1995. Sharon Brookes G. Alistair Lammie et al , Linkage map of a region of human chromosome band 11q13 amplified in breast and squamous cell tumors, Genes Chromosomes & Cancer, Volume 4, Issue 4 June 1992 Pages 290-301.Jiulia I.Perinia ,Elisabetta ,Menegazzo et al; Cognitive impairment and (CTG)n expansion in myotonic dystrophy patients Biological Psychiatry Volume 46, Issue 3, 1 August 1999, Pages 425-431.Ralph Carvalho, Anya N. A. Milne et al, A novel region of amplification at 11p12-13 in gastric cancer, revealed by representational difference analysis, is associated with overexpression of CD44v6, especially in early-onset gastric carcinomas, Genes Chromosomes & Cancer, Volume 45, Issue 10 October 2006 Pages 967-975.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200