Enzymes for Molecular Biology: Enzymes for Molecular Biology
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase catalyses the transfer of deoxynucleoside monophosphate from deoxynucleoside triphosphate to 3"-OH termini of DNA molecules or oligonucleotide initiators
Cambio
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| PM-1390-01 | Terminal Deoxynucleotidyl Transferase | 300U | £105.00 | Quantity | Add to Order |
| PM-1390-02 | Terminal Deoxynucleotidyl Transferase | 1,500U | £240.00 | Quantity | Add to Order |
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase catalyses the transfer of deoxynucleoside monophosphate from deoxynucleoside triphosphate to 3"-OH termini of DNA molecules or oligonucleotide initiators
Cambio
Applications
- Preferred substrates are single-stranded DNA, double-stranded DNA with 3’-hydroxyl termini and oligodeoxynucleotide primers (1)
- Used for specific labelling of 3"-termini with ribonucleotides (2)
- Labels 3"-ends of DNA fragments with an [α-32P] 3"-deoxynucleoside (3)
- Adds homopolymer tails of deoxyribonucleotides to vectors or cDNAs (4,5)
Reagents Supplied
5X Tdt C-Tailing Reaction Buffer
Unit Definition
One unit is the amount of enzyme required to transfer 1 nmol of dAMP from dATP to the 3" OH terminus of the oligonucleotide initiator d(A)15 for 1 hour at 37ºC
Heat Inactivation
15 minutes at75ºC
Concentration
10-15 units/µl
Storage Buffer
50 mM potassium phosphate (pH 7.4)1 mM β-mercaptoethanol50% (w/v) glycerol
Assay Conditions
40 mM K Cacodylate (pH 7.2)8 mM MgCl20.33 mM ZnSO410 µM oligonucleotide d(A)5010 µlbovine serum albumin buffer1 mM (α-32P) dATP Reaction volume of 60 µl
Note: All reactions should be run in polypropylene tubes
Storage Conditions
Store at -20°C
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase catalyses the transfer of deoxynucleoside monophosphate from deoxynucleoside triphosphate to 3"-OH termini of DNA molecules or oligonucleotide initiators
Cambio
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase catalyses the transfer of deoxynucleoside monophosphate from deoxynucleoside triphosphate to 3"-OH termini of DNA molecules or oligonucleotide initiators
Cambio
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
