Enzymes for Molecular Biology: Enzymes for Molecular Biology
RNA Poly A Polymerase
Poly A RNA polymerase adds poly A tails to the 3? hydroxyl terminus of RNA and is useful for cloning purposes. 3?-end labelling RNA with a[32P]-cordycepin triphosphate and determining molar concentration of polyadenylated RNA. All preparations are assayed for contaminating ribonuclease activity
Cambio
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| PM-1240-01 | RNA Poly A Polymerase | 40U | £105.00 | Quantity | Add to Order |
| PM-1240-02 | RNA Poly A Polymerase | 200U | £240.00 | Quantity | Add to Order |
Related products
RNA Poly A Polymerase
Poly A RNA polymerase adds poly A tails to the 3? hydroxyl terminus of RNA and is useful for cloning purposes. 3?-end labelling RNA with a[32P]-cordycepin triphosphate and determining molar concentration of polyadenylated RNA. All preparations are assayed for contaminating ribonuclease activity
Cambio
Source
Escherichia coli
Applications
- Adds poly A tails to the 3’-hydroxyl terminus of RNA (1)
- Labels 3’-ends of RNA with a [α-32P] cordycepin triphosphate (2)
- Ideal for determining molar concentration of polyadenylated RNA (3)
Unit Definition
One unit is the amount of enzyme required to incorporate 1.0 nmol of labeled ATP into acid insoluble form for 10 minutes at 37°Cunder the stated assay conditions
Concentration
1 Unit/µl
Storage Buffer
25 mM Tris-HCl (pH 7.9)0.1 mM DTT0.01% (v/v) Triton X-100™1.0 mM EDTA0.5 M NaCl50% (v/v) glycerol
Assay Conditions
50 mM Tris-HCl (pH 7.9)10 mM MgCl22.5 mM MnCl2250 mM NaCl25 µg tRNA0.1 mM [α-32P] ATP50 μg bovine serum albuminReaction volume of 100 µl
Storage Conditions
Store at -20°C
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNA Poly A Polymerase
Poly A RNA polymerase adds poly A tails to the 3? hydroxyl terminus of RNA and is useful for cloning purposes. 3?-end labelling RNA with a[32P]-cordycepin triphosphate and determining molar concentration of polyadenylated RNA. All preparations are assayed for contaminating ribonuclease activity
Cambio
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
RNA Poly A Polymerase
Poly A RNA polymerase adds poly A tails to the 3? hydroxyl terminus of RNA and is useful for cloning purposes. 3?-end labelling RNA with a[32P]-cordycepin triphosphate and determining molar concentration of polyadenylated RNA. All preparations are assayed for contaminating ribonuclease activity
Cambio
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
