Cell culture contamination control: Detection
Detection and identification of Mycoplasma
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 11-7024 | Venor®GeM Advance Mycoplasma Detection Kit | 24 reactions | £148.00 | Quantity | Add to Order |
| 11-7048 | Venor®GeM Advance Mycoplasma Detection Kit | 48 reactions | £271.00 | Quantity | Add to Order |
| 11-7096 | Venor®GeM Advance Mycoplasma Detection Kit | 96 reactions | £472.00 | Quantity | Add to Order |
| 11-7240 | Venor®GeM Advance Mycoplasma Detection Kit | 240 reactions | £1,004.00 | Quantity | Add to Order |
Related products
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
Venor®GeM Advance
Venor®GeM Advance contains PCR reaction tubes pre-coated with all PCR reagents to reduce the total assay time without need to prepare aliquots of a master mix. For additional convenience the gel loading buffer and dye are already included in the reaction buffer. After thermal cycling the PCR can be loaded directly on the agarose gel.
Type of PCR
For conventional, endpoint PCR
Scope of Application
Applicable in research for direct testing of cell cultures and cell culture-derived biologicals. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.
Kit Components
Primer sets, nucleotides, polymerase and internal control DNA, lyophilized, filled in 0.2 ml PCR reaction tubes Rehydration buffer including gel loading buffer and running dye Positive controls, lyophilized, filled in 0.2 ml PCR reaction tubes PCR grade water
Package Sizes
Cat. No. 11-7024 24 Tests
Cat. No. 11-7048 48 Tests
Cat. No. 11-7096 96 Tests
Cat. No. 11-7240 240 Tests
Result evaluation
Gel electrophoresis at endpoint of PCRRequired Consumables
Agarose and DNA-specific dye for gel staining
Required lab devices
Regular block-heated PCR cycler Agarose gel electrophoresis and gel documentation system Pipetting equipment Tube centrifuge
Shelf Life and Storage
Kit components are maintainable at2 to8 °C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.
EP 2.6.7 compliance
No
Fig. Amplified PCR products are visualized by standard gel electrophoresis.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
Venor®GeM Advance protocol
Quick Visual protocol
Please note: all protocols off site are the responsibility of the products supplier
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
Click here to view a list of citations
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
Required consumables:
Agarose and DNA-specific dye for gel staining
Required lab devices:
Regular block-heated PCR cyclerAgarose gel electrophoresis and gel documentation systemPipetting equipmentTube centrifuge
Shelf life and storage:
Kit components are maintainable at2 to8 °C for at least 6 months. After rehydratisation of the individual PCR tubes the reagents must be used within 1 hour.
EP 2.6.7 compliance:
No
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
Application
For the rapid and reliable detection of mycoplasma in various in situ biologicals including cell cultures and virus stocks
Benefits
- Sensitive - Detection of 5 copies/µl of mycoplasma DNA that corresponds to 10 mycoplasma genomes per sample volume.
- Specific - The kit is specific for more than 79 mycoplasma species including all relevant species mentioned in the Eur. Ph. 2.6.7. For the detection of the avian associated mycoplasma species M. synoviae and M. gallisepticum the product Venor®MGS is highly recommended. Eukaryotic and bacterial DNA is not amplified.
- Reliable - The tubes contain internal control DNA for individual inhibition control. The pre-aliquoted reagents optimize reproducibility and minimize operator-dependent variability of results.
- Simple - Only rehydration buffer, polymerase and sample have to be added to the PCR tube.
- Clear - A clear and easily interpretable result is obtained with one PCR reaction.
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Venor®GeM Advance Mycoplasma Detection Kit
Venor®GeM Advance is a ready-to-go version of Venor®GeM. The reaction tubes included with the kit are pre-coated with appropriate dNTPs and primers to reduce the total assay time compared to the general protocol that requires individual loading of reaction tubes. For additional convenience the gel loading buffer and dye are already included in the reaction tubes. After thermal cycling the PCR can be loaded directly on the agarose gel.
Minerva Biolabs
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
- Venor™GeM; Frequently Asked Questions
- Troubleshooting: PCR & qPCR kits
- Venor™GeM Advance Protocol
