Transfection - EZ Biosystems: Cell Lines

Cell Line Information:

Designations: Vero Organism:Cercopithecus aethiops Morphology:epithelial Source:Organ: kidney Disease: normal Isolation date: March 27, 1962 Virus Resistance: Stratford; Apeu; Caraparu; Madrid; Nepuyo; Ossa. Age: adult

Applications:

• detection of verotoxin
• efficacy testing
• malaria biology
• media testing
• mycoplasma testing
• transfection host
• detection of virus in ground beef.

The Vero cell line was initiated from the kidney of a normal adult African green monkey on March 27, 1962, by Y. Yasumura and Y. Kawakita at the Chiba University in Chiba, Japan.

The Transfection Reagent:

Vero Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Vero Cells. The proprietary formulation of lipids and polymers ensures the maximum possible transfection efficiency and cell viability.No other transfection reagents can match the efficiency, convenience, and gentleness of Vero Cell Avalanche™ Transfection Reagent for transfection on Vero Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Highest efficiency to ensure experimental success
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Simplest and straightforward protocols
• Suitable for Reverse Transfection
• Compatible with transfection in any plate formats
• Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on Vero Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for Vero Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this Vero Cell Avalanche Transfection Reagent. FIG. 2. Vero cells were transfected with GFP vector (pEGFP-N3) by using Vero Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.

For Other Cells:

Vero Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies. 786-O Cell Caki-1 Cell MDCK Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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