Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell)
ransfection of suspension cells, such as U-937 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.U-937 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on U-937 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U-937 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of U-937 Cell Avalanche™ Transfection Reagent for transfection on U-937 Cells.
Aline Biosciences
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| EZT-U937- | U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
| EZT-U937-1-15 | U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell)
ransfection of suspension cells, such as U-937 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.U-937 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on U-937 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U-937 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of U-937 Cell Avalanche™ Transfection Reagent for transfection on U-937 Cells.
Aline Biosciences
| Catalog Number:EZT-U937-1 | |||
| Cell Synonyms:CRL-1593.2 , U937, U 937 | |||
Cell Line Information: Designations: U-937Morphology: monocyteSource: histiocytic lymphomaCulture Properties: suspensionCellular Products: lysozyme; beta-2-microglobulin (beta 2 microglobulin); tumor necrosis factor (TNF) after stimulation with phorbol myristic acid (PMA)Age: 37 yearsGender: maleEthnicity: CaucasianThe U-937 cell line was derived by Sundstrom and Nilsson in 1974 from malignant cells obtained from the pleural effusion of a patient with histiocytic lymphoma.Studies since 1979 have shown that U-937 cells can be induced to terminal monocytic differentiation by supernatants from human mixed lymphocyte cultures,phorbol esters, vitamin D3, gamma interferon, tumor necrosis factor (TNF) and, retinoic acid. The cells express the Fas antigen, and are sensitive to TNF and anti-Fas antibodies. The Transfection Reagent: Transfection of suspension cells, such as U-937 Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments. U-937 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on U-937 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for U-937 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of U-937 Cell Avalanche™ Transfection Reagent for transfection on U-937 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
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| U-937 Cell Avalanche™ Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies. 293 Cell293T/17 CellNIH/3T3 Cell3T3-L1 CellBALB/3T3 clone A313T3-Swiss albino CellCT26.WT CellNCTC Clone 929 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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