Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
U251 Cell Avalanche® Transfection Reagent (human glioblastoma cell
U251 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U251 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U251 cells
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
|
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EZT-U251-1-05 | U251 Cell Transfection Reagent (human glioblastoma cell | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-U251-1-15 | U251 Cell Transfection Reagent (human glioblastoma cell | 1.5 ml | £699.00 | Quantity | Add to Order |
U251 Cell Avalanche® Transfection Reagent (human glioblastoma cell
U251 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U251 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U251 cells
EZ Biosystems
Cell Line Information: Cell Line Name: U251 Patient"s Age: 75 Patient"s Sex: M Tissue Type: CNS Histology: Glioblastoma-ud; non epithelial, Pleomorphic/astrocytoid p53 Status: 0 ECAD Methylation: 11 MDR Function: -19 Institution: Uppsala University, Sweden Reference: Med Biol. 56:184-193, 1978 Biological source: Brain from human growth mode: Adherent karyotype: 2n = 46 receptors: PDGFR alpha and EGFR. See Nistér M. et al., (1988) & (1991) Comments: Derived from a malignant glioblastoma tumour by explant technique. U-251 was formerly distributed as U-373 MG (Sigma Catalogue number 89081403) until short tandem repeat (STR)-PCR profiling confirmed identity with U-251. SNB19 are derived with the same individual with U251. U251 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.The Transfection Reagent: U251 Cell Avalanche® Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting U251 cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for U251 cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche® Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in U251 cells
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Free of animal-derived components
- Simplest and straightforward protocols to Increase experimental throughput
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate format
- Great pricing
- Developed and manufactured by EZ Biosystems
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U251 Cell Avalanche® Transfection Reagent (human glioblastoma cell) can also be used on the following cells with high transfection efficiencies. SF268 Cell SF295 Cell SNB75 Cell SF539 Cell MRC-5 Cell IMR-90 Cell COS-7 Cell BHK-21 Cell 3197-3 Cell NIH/3T3 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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