Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
T84 Cell Avalanche™ Transfection Reagent (human colon carcinoma cell)
T84 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on T84 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for T84 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of T84 Cell Avalanche™ Transfection Reagent for transfection on T84 Cells.
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-T840-1-05 | T84 CellTransfection Reagent (human colon carcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-T840-1-15 | T84 CellTransfection Reagent (human colon carcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
T84 Cell Avalanche™ Transfection Reagent (human colon carcinoma cell)
T84 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on T84 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for T84 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of T84 Cell Avalanche™ Transfection Reagent for transfection on T84 Cells.
EZ Biosystems
ell Line Information:
Designations: T84 Depositors: H Masui Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: colon Disease: colorectal carcinoma Derived from metastatic site: lung Cellular Products: carcinoembryonic antigen (CEA), 600 ng/ml per 10 exp6 cells per 10 days; keratin Applications: transfection host Receptors: neurotransmitter, peptide hormone Tumorigenic: Yes Age: 72 year Gender:male Comments: The T84 cell line is a transplantable human carcinoma cell line derived from a lung metastasis of a colon carcinoma in a 72-year-old male. After 23 passages in athymic mice, the T84 cell line was established. These cells grow to confluence as monolayers and exhibit tight junctions and desmosomes between adjacent cells. They have receptors for many peptide hormones and neurotransmitters and maintain vectorial electrolyte transport. This line exhibits tight junctions, and desmosomes between adjacent cells. The cells are positive for keratin by immunoperoxidase staining.
The Transfection Reagent:
T84 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on T84 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for T84 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of T84 Cell Avalanche™ Transfection Reagent for transfection on T84 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in T84 Cells.
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Deliver single or multiple plasmids
- Efficient for plasmid DNA/siRNA co-transfection
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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T84 Cell Avalanche™ Transfection Reagent (human colon carcinoma cell) can also be used on the following cells with high transfection efficiencies. HCT 116 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW480 Cell SW620 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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