Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
SW620 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
SW620 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SW620 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SW620 Cells
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-SW62-1-05 | SW620 Cell Transfection Reagent (human colon adenocarcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-SW62-1-15 | SW620 Cell Transfection Reagent (human colon adenocarcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
SW620 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell)
SW620 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SW620 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SW620 Cells
EZ Biosystems
Cell Line Information:
Designations: SW620 [SW-620] Depositors: A Leibovitz Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: colon Tumor Stage: Dukes" type C Disease: colorectal adenocarcinoma Derived from metastatic site: lymph node Cellular Products: carcinoembryonic antigen (CEA) 0.15 ng/10 exp6 cells/10 days; transforming growth factor alpha; keratin; matrilysin Applications: transfection host Tumorigenic: Yes Oncogene: myc; myb ; ras; fos; sis; p53; abl -; ros -; src - Antigen Expression: blood type A; RhAge: 51 years Gender:male Ethnicity: Caucasian Comments: SW620 was isolated from the tissue of a 51-year-old Caucasian male (blood group A, Rh ). A recurrence of the malignancy resulted in a wide-spread metastasis from the colon to an abdominal mass. The established cell line consists mainly of individual small spherical and bipolar cells lacking microvilli. There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
SW620 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
SW620 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting SW620 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for SW620 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into SW620 Cells
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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SW620 Cell Avalanche™ Transfection Reagent (human colon adenocarcinoma cell) can also be used on the following cells with high transfection efficiencies. T84 Cell HCT 116 Cell DLD-1 Cell HT-29 Cell Caco-2 Cell LS 174T Cell LoVo Cell SW480 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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