Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
RPMI 8226 Cell Avalanche™ Transfection Reagent (human myeloma cell)
Transfection of suspension cells, such as RPMI 8226, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.RPMI 8226 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RPMI 8226. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RPMI 8226
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-RPMI-1-05 | RPMI 8226 Cell ransfection Reagent (human myeloma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-RPMI-1-15 | RPMI 8226 Cell ransfection Reagent (human myeloma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
RPMI 8226 Cell Avalanche™ Transfection Reagent (human myeloma cell)
Transfection of suspension cells, such as RPMI 8226, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.RPMI 8226 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RPMI 8226. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RPMI 8226
EZ Biosystems
Cell Line Information: Designations: RPMI 8226 Organism: Homo sapiens (human) Tissue: plasmacytoma; myeloma; B lymphocyte Age: 61 years Gender: male Morphology: lymphoblast Growth properties: suspension Isotype: lambda light chain VirusSuscept: poliovirus 1; vesicular stomatitis (Indiana); herpes simplex; vaccinia Reverse Transcriptase: negative Isoenzymes: G6PD, A AntigenExp: HLA Aw19, B15, B37, Cw2; CD19 -; CD20 -; CD28; CD38; CD49eThere is no evidence of heavy chain production (cytoplasmic or secreted) Depositors: G.E. Moore
RPMI 8226 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
Transfection of suspension cells, such as RPMI 8226, is a problem in the laboratory routine and further in tissue engineering and gene therapy. Most methods working effectively for adherent cells in culture fail to transfect suspension cells.
RPMI 8226 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RPMI 8226. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for RPMI 8226. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Highest efficiency to ensure experimental success
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Animal-free
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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For Other Cells: | |||
RPMI 8226 Cell Avalanche™ Transfection Reagent (human myeloma cell) can also be used on the following cells with high transfection efficiencies. MDCK Cell Vero Cell 293 Cell 293T/17 Cell MG-63 Cell WI-38 Cell COS-7 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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