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商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-RBL2-1-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-RBL2-1-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-RBL2-1-05
商品编号: EZT-RBL2-1-05
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产地: 美国(厂家直采)
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产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
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商品介绍
Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell)

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
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  • €
EZT-RBL2-1-05RBL-2H3 CellTransfection Reagent (rat leukemia cell)0.5ml£399.00QuantityAdd to Order
EZT-RBL2-1-15RBL-2H3 CellTransfection Reagent (rat leukemia cell)1.5ml£699.00QuantityAdd to Order

RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell)

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells

EZ Biosystems

Cell Line Information:

Designations: RBL-2H3 Depositors: RP Siraganian Growth Properties: adherent Organism: Rattus norvegicus deposited as Rattus sp. Morphology: fibroblast Source: peripheral blood Strain: Wistar Disease: basophilic leukemia Cell Type: basophil; chemically induced Cellular Products: histamine Applications: transfection host Receptors: FcERI (Fc of IgE) Comments: RBL-2H3 is a basophilic leukemia cell line isolated and cloned in 1978 from Wistar rat basophilic cells that were maintained as tumors. These cells have high affinity IgE receptors. They can be activated to secrete histamine and other mediators by aggregation of these receptors or with calcium ionophores. They have been used extensively to study FcERI and the biochemical pathways for secretion in mast cells. They have also been used extensively for studies of different aspects of secretion in cells including the role of changes in intracellular calcium, the activation of phospholipases, protein kinases and small G proteins.

The Transfection Reagent:

RBL-2H3 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting RBL-2H3 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for RBL-2H3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in RBL-2H3 Cells.
  • Extremely gentle to cells
  • Deliver single or multiple plasmids
  • Simplest and straightforward protocols
  • Compatible with serum
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RBL-2H3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RBL-2H3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RBL-2H3 Cell Avalanche Transfection Reagent.
For Other Cells:
RBL-2H3 Cell Avalanche™ Transfection Reagent (rat leukemia cell) can also be used on the following cells with high transfection efficiencies.

786-O Cell Caki-1 Cell MDCK Cell Vero Cell 293 Cell 293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。