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当前位置: 首页 > 产品中心 > peptide > Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-PC30-1-05
商品详细Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-PC30-1-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-PC30-1-05
Athens Research/Cambio - Excellence in Molecular Biology/0.5ml/EZT-PC30-1-05
商品编号: EZT-PC30-1-05
市场价: ¥0.00
美元价: 0.00
产地: 美国(厂家直采)
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产品分类: 多肽合成
公司分类: peptide
联系Q Q: 3392242852
电话号码: 4000-520-616
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商品介绍
Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-PC30-1-05PC-3 Cell Avalanche™ Transfection Reagent0.5ml£399.00QuantityAdd to Order
EZT-PC30-1-15PC-3 Cell Avalanche™ Transfection Reagent1.5ml£699.00QuantityAdd to Order

PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell)

EZ Biosystems

Cell Line Information:

Designations: PC-3 Organism: Homo sapiens (human) Tissue: adenocarcinoma; prostate; metastatic site: bone Age: 62 years Tumor Stage: grade IV Gender: male Ethnicity: Caucasian Morphology: epithelial Growth properties: adherent Tumorigenic: yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells AntigenExp: HLA A1, A9 Growth Properties: monolayer; the cells form clusters in soft agar and can be adapted to suspension growth Depositors: M.E. Kaighn The PC-3 was initiated from a bone metastasis of a grade IV prostatic adenocarcinoma from a 62-year-old male Caucasian. The cells exhibit low acid phosphatase and testosterone-5-alpha reductase activities.

PC-3 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.

The Transfection Reagent:

PC-3 Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on PC-3 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for PC-3 Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of PC-3 Cell Avalanche™ Transfection Reagent for transfection on PC-3 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

References:

Akamatsu S, Wyatt AW, Lin D, Lysakowski S, Zhang F, et al. The Placental Gene PEG10 Promotes Progression of Neuroendocrine Prostate Cancer.Cell Rep. 2015 Aug 11;12(6):922-36. PubMed PMID: 26235627.

Features:

  • Specifically optimized to deliver nucleic acids into PC-3 Cells
  • Highest efficiency to ensure experimental success
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • 0.5 ml is able to transfect about 1000 wells of 24-well plate
  • Deliver single or multiple plasmids
  • Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
  • Compatible with serum
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on PC-3 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for PC-3 cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this PC-3 Cell Avalanche Transfection Reagent.
FIG. 2. PC-3 cells were transfected with GFP vector (pEGFP-N3) by using PC-3 Cell Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
PC-3 Cell Avalanche™ Transfection Reagent (human prostate carcinoma cell) can also be used on the following cells with high transfection efficiencies.

DU 145 Cell 22Rv1 Cell VCaP Cell Vero Cell 293 Cell 293T/17 Cell Calu-3 Cell Caki-1 Cell MDCK Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.
Research Use Only. Not for use in diagnostic or therapeutic procedures.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。