Transfection - EZ Biosystems: Cell Lines

Designations: Raji Depositors: W Henle Growth Properties: suspension Organism: Homo sapiens Morphology:lymphoblast Source: Burkitt"s lymphoma Cell Type: B lymphocyte; Isolation date: 1963 Applications: transfection host Virus Resistance: The cells are partially resistant to poliovirus and vesicular stomatitis viruses. Age: 11 years Gender:male Ethnicity: Black Comments: The Raji line of lymphoblast-like cells was established by R.J.V. Pulvertaft in 1963 from a Burkitt"s lymphoma of the left maxilla of an 11-year-old Black male. EBNA positive.

The Transfection Reagent:

Transfection of suspension cells, such as Raji Cells, has always been challenging, both technically and financially. The most common systems currently used for relatively efficient molecular delivery to these cells are expensive electroporation instruments.

Raji Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting Raji Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for Raji Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Unique formulation-maximize transfection performance in Raji Cells.
• Extremely gentle to cells
• Deliver single or multiple plasmids
• Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum
• Compatible with transfection in any plate formats
• Economical: High efficiency means less amount of nucleic acid & reagent is needed
• Developed and manufactured by EZ Biosystems

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on RAJI cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for RAJI cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this RAJI Cell Avalanche Transfection Reagent.

For Other Cells:

Raji Cell Avalanche™ Transfection Reagent (human lymphoma cell) can also be used on the following cells with high transfection efficiencies. Vero Cell 293 Cell VCaP Cell PC-3 Cell AR42J Cell Capan-1 Cell PANC-1 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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