Transfection - EZ Biosystems: Cell Lines

Cell Line Information:

Designations: NCI-H460 Depositors: AF Gazdar, JD Minna Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Organ: lung Disease: carcinoma; large cell lung cancer Derived from metastatic site: pleural effusion Isolation: Isolation date: 1982 Tumorigenic: Yes Gender: male The NCI-H460 cell line was derived by A.F. Gazdar and associates in 1982 from the pleural fluid of a patient with large cell cancer of the lung. The cells express easily detectable p53 mRNA at levels comparable to normal lung tissue, and exhibit no gross structural DNA abnormalities. The cells stain positively for keratin and vimentin but are negative for neurofilament triplet protein.

The Transfection Reagent:

NCI-H460 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H460 Cells.The proprietary formulation of lipids and polymers ensures the maximum posssible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H460 Cell Avalanche™ Transfection Reagent for transfection on NCI-H460 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

• Specifically optimized to deliver nucleic acids into NCI-H460 Cells
• Highest efficiency to ensure experimental success
• Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
• Deliver single or multiple plasmids
• Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
• Compatible with serum

Data:

FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on A549 cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for A549 cells. This test result was confirmed with repeat experiments. The candidate formulas were also tested on NCI-H460 cells by tranfecting GFP expression plasmid, and got the same high transfection efficiency after flow cytometry analysis. The one that showed the optimal balance of potent & low cytotoxicity (percentage of 7AAD positive cells) among those candidate formulas was later named as NCI-H460 Cell Avalanche Transfection Reagent.

For Other Cells:

NCI-H460 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. A549 Cell, NCI-H292 Cell, NCI-H1299 Cell, NCI-H23 Cell, NCI-H1975 Cell, NCI-H441 Cell, Calu-3 Cell, NCI-H358 Cell, 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

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