Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
NCI-H358 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell)
NCI-H358 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H358 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H358 Cells
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-NCIH-7-05 | NCI-H358 Cell Transfection Reagent (human lung carcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-NCIH-7-15 | NCI-H358 Cell Transfection Reagent (human lung carcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
NCI-H358 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell)
NCI-H358 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H358 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for NCI-H358 Cells
EZ Biosystems
Cell Line Information:
Designations: NCI-H358 [H-358, H358] Depositors: AF Gazdar, JD Minna Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source:Organ: lung Tissue: bronchiole; alveolus Disease: bronchioalveolar carcinoma; non-small cell lung cancer Cellular Products: lung surfactant associated protein A (SP-A) Isolation date: 1981 Tumorigenic: Yes Gender:male Comments: This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates in 1981 from tumor tissue obtained from a patient prior to initiation of chemotherapy. Ultrastructural studies demonstrated the presence of cytoplasmic structures characteristic of Clara cells. The cells expressed protein and RNA of SP-A, the major lung surfactant associated protein.
The Transfection Reagent:
NCI-H358 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H358 Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for NCI-H358 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in NCI-H358 Cells.
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Simplest and straightforward protocols
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
- Developed and manufactured by EZ Biosystems
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For Other Cells: | |||
NCI-H358 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. A549 Cell NCI-H292 Cell NCI-H460 Cell NCI-H1299 Cell NCI-H23 Cell NCI-H1975 Cell NCI-H441 Cell Calu-3 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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