Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
NCI-H1299 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell)
NCI-H1299 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H1299 Cells. The proprietary formulation of lipids and polymers ensures the maximum posssible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H1299 Cell Avalanche™ Transfection Reagent for transfection on NCI-H1299 Cells.
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-NCIH-4-05 | NCI-H1299 Cell Transfection Reagent (human lung carcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-NCIH-4-15 | NCI-H1299 Cell Transfection Reagent (human lung carcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
NCI-H1299 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell)
NCI-H1299 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H1299 Cells. The proprietary formulation of lipids and polymers ensures the maximum posssible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H1299 Cell Avalanche™ Transfection Reagent for transfection on NCI-H1299 Cells.
EZ Biosystems
Cell Line Information:
Designations: NCI-H1299 Organism: Homo sapiens (human) Tissue: lung carcinoma; non-small cell lung cancer; derived from metastatic site: lymph node Age: 43 years Gender: male Ethnicity: Caucasian Morphology: epithelial Growth properties: adherent Products: neuromedin B (NMB) Depositors: A.F. Gazdar; J.D. Minna This line was derived by A.F. Gazdar, H.K. Oie, J.D. Minna and associates from a lymph node metastasis. The cells have a homozygous partial deletion of the p53 protein, and lack expression of p53 protein. The cells are able to synthesize the peptide NMB at 0.1 pmol/mg protein, but not the gastrin releasing peptide (GRP).
The Transfection Reagent:
NCI-H1299 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting NCI-H1299 Cells.The proprietary formulation of lipids and polymers ensures the maximum posssible transfection efficiency and cell viability. No other transfection reagents can match the efficiency, convenience, and gentleness of NCI-H1299 Cell Avalanche™ Transfection Reagent for transfection on NCI-H1299 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Unique formulation-maximize transfection performance in NCI-H1299 Cells.
- Extremely gentle to cells
- Deliver single or multiple plasmids
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Compatible with transfection in any plate formats
- Economical: High efficiency means less amount of nucleic acid & reagent is needed
- Developed and manufactured by EZ Biosystems
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For Other Cells: | |||
NCI-H1299 Cell Avalanche™ Transfection Reagent (human lung carcinoma cell) can also be used on the following cells with high transfection efficiencies. A549 Cell NCI-H292 Cell NCI-H460 Cell NCI-H23 Cell NCI-H1975 Cell NCI-H441 Cell Calu-3 Cell NCI-H358 Cell 293 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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