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商品详细雅典研究院/坎比奥-卓越分子生物学/1.5ml/EZT-MDCK-1-15
雅典研究院/坎比奥-卓越分子生物学/1.5ml/EZT-MDCK-1-15
雅典研究院/坎比奥-卓越分子生物学/1.5ml/EZT-MDCK-1-15
商品编号: EZT-MDCK-1-15
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产品分类: 多肽合成
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商品介绍
Transfection - EZ Biosystems

Transfection - EZ Biosystems: Cell Lines

Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:

Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.

For primary cells click HERE

To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE

  • Catalogue
  • Description

MDCK Cell Avalanche™ Transfection Reagent

MDCK Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MDCK Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDCK Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MDCK Cell Avalanche™ Transfection Reagent for transfection on MDCK Cells.

EZ Biosystems

Catalogue No.DescriptionPack SizePriceQty
  • Change to:
  • €
EZT-MDCK-1-05MDCK Cell Transfection Reagent0.5ml£399.00QuantityAdd to Order
EZT-MDCK-1-15MDCK Cell Transfection Reagent1.5ml£699.00QuantityAdd to Order

MDCK Cell Avalanche™ Transfection Reagent

MDCK Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MDCK Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDCK Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MDCK Cell Avalanche™ Transfection Reagent for transfection on MDCK Cells.

EZ Biosystems

Cell Line Information:

Designations: MDCK Cellular Products:keratin Isolation date: September, 1958 Applications:transfection host Virus Susceptibility:Human Coxsackievirus B, Human Coxsackievirus B, Human Coxsackievirus B, Human poliovirus. Age:adult Gender:female Comments:The MDCK cell line was derived from a kidney of an apparently normal adult female cocker spaniel, September, 1958, by S.H. Madin and N.B. Darby. The cells are positive for keratin by immunoperoxidase staining. MDCK cells have been used to study processing of beta amyloid precursor protein and sorting of its proteolytic products.

The Transfection Reagent:

MDCK Cell Avalanche™ Transfection Reagent is a new, proprietary solution specifically designed for transfection on MDCK Cells. The proprietary formulation of lipids and polymersensures the highest possible transfection efficiencies and viabilities for MDCK Cells. No other transfection reagents can match the efficiency, convenience, and gentleness of MDCK Cell Avalanche™ Transfection Reagent for transfection on MDCK Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?

Features:

  • Unique formulation-maximize transfection performance in MDCK Cells.
  • Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
  • Deliver single or multiple plasmids
  • Easy-to-use protocol: Just mix nucleic acids with the reagent to form complexes and simply add the mixture to cells
  • Suitable for Reverse Transfection
  • Compatible with transfection in any plate formats
  • Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
  • Economical: High efficiency means less amount of nucleic acid & reagent is needed
  • Developed and manufactured by EZ Biosystems
Data:
FIG. 1. High throughput test of transfection efficiency (determined as RLU/mg) on MDCK Cells after transfection of luciferase reporter gene by using our 172 proprietary transfection formulas and several most popular commercial transfection reagents. The yellow box showed the results of 4 commercial transfection reagents. The red lines marked our candidate formulas with the highest transfection efficiency for MDCK Cells. This test result was confirmed with repeat experiments. The one that showed the optimal balance of potent & low cytotoxicity among those candidate formulas after flow cytometry analysis on the percentage of 7AAD positive cells was later named as this MDCK Cell Avalanche Transfection Reagent.
FIG. 2. MDCK cells were transfected with GFP vector (pEGFP-N3) by using MDCK Avalanche® Transfection Reagent. The cells were visualized by Nikon Eclipse Fluorescence microscope 24 hours post transfection.
For Other Cells:
MDCK Cell Avalanche™ Transfection Reagent can also be used on the following cells with high transfection efficiencies.

786-O Cell Caki-1 Cell Vero Cell 293 Cell 293T/17 Cell

Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list.

If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200

品牌介绍
Athens Research & Technology—供应多款蛋白、酶制品 Athens Research & Technology是成立于2010年的新兴生物高科技制品公司,该公司拥有BSL-2实验室及超过11000平方英尺的cGMP生产厂房,并且通过ISO 9001:2008认证。Athens公司致力于纯化分离高纯度,高活性的人类蛋白质及研发多克隆抗血清产品。提供包括高纯度及活性的丝氨酸蛋白酶,蛋白酶抑制剂,中性粒细胞酶,载脂蛋白,脂蛋白,血小板蛋白,转铁蛋白,免疫球蛋白等等。公司产品适用于炎症,冠状动脉疾病,自身免疫性疾病,癌症,阿尔茨海默氏病等众多研究领域,已被世界*制药公司及诊断试剂公司用于体外诊断试剂盒/免疫检测试剂盒、药物筛选、细胞培养液(包括干细胞)等产品研发。除了人类蛋白质,我们还从动物血清和组织中分离多种蛋白质及酶类。此外,Athens Research and Technology还提供特殊试剂/蛋白定制服务,以满足研究人员的不同需求。