Transfection - EZ Biosystems: Cell Lines
Avalanche®-Cell Type / Cell Line-specific Transfection Reagent series:
Perform maximum transfection efficiency for more than 170 different primary cells and cell lines including 124 cancer cell lines (e.g. All of NCI60 cancer cell lines).Each of the cell type/cell line-specific transfection reagents in our Avalanche® series was individually formulated.
For primary cells click HERE
To learn why you should use Avalanche®Cell Type-Specific Transfection reagents, clickHERE; To learn how EZ Biosystems developed Avalanche®Cell Type-Specific Transection Reagents, clickHERE
MDA-MB-231 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell)
MDA-MB-231 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting MDA-MB-231 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDA-MB-231 Cells.
EZ Biosystems
Catalogue No. | Description | Pack Size | Price | Qty |
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EZT-MDAM-1-05 | MDA-MB-231 Cell Transfection Reagent (human breast carcinoma cell) | 0.5ml | £399.00 | Quantity | Add to Order |
EZT-MDAM-1-15 | MDA-MB-231 Cell Transfection Reagent (human breast carcinoma cell) | 1.5ml | £699.00 | Quantity | Add to Order |
MDA-MB-231 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell)
MDA-MB-231 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting MDA-MB-231 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDA-MB-231 Cells.
EZ Biosystems
Cell Line Information:
Designations: MDA-MB-231 Depositors: R Cailleau Growth Properties: adherent Organism: Homo sapiens Morphology: epithelial Source: mammary gland; breast Disease: adenocarcinoma Derived from metastatic site: pleural effusion Cell Type: epithelial Applications: transfection host Receptors: epidermal growth factor (EGF), Transforming growth factor alpha (TGF alpha) Tumorigenic: Yes Age: 51 years adult Gender:female Ethnicity: Caucasian Comments: The cells express the WNT7B oncogene [PubMed: 8168088].
MDA-MB-231 is one of the cell lines of the NCI-60 panel which represents different cancer types and has been widely utilized for drug screening and molecular target identification.
The Transfection Reagent:
MDA-MB-231 Cell Avalanche™ Transfection Reagent is a new class of unique chemical formulations specifically formulated and optimized for transfecting MDA-MB-231 Cells. The proprietary formulation of lipids and polymers ensures the highest possible transfection efficiencies and viabilities for MDA-MB-231 Cells. For details of the developing process of this reagent, please go to: How did EZ Biosystems develop Avalanche™ Cell type/cell line specific transfection reagent series?
Features:
- Specifically optimized to deliver nucleic acids into MDA-MB-231 Cells
- Highest efficiency to ensure experimental success
- Lowest Cellular Toxicity-maintain cell density and reduce experimental biases
- Efficient for plasmid DNA/siRNA co-transfection
- Synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in research experiments or cells lines
- Simplest and straightforward protocols
- Compatible with serum
- Suitable for Reverse Transfection
- Reproducible: due to highly controlled chemical synthesis of each of the ingredients, the reagent forms uniformly sized complex particles with nucleic acids. With optimized protocol, our reagent will ensure the reproducible highest transfection results.
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MDA-MB-231 Cell Avalanche™ Transfection Reagent (human breast carcinoma cell) can also be used on the following cells with high transfection efficiencies. 4T1 Cell ZR-75-1 Cell BT-20 Cell MDA-MB-453 Cell HCC1937 Cell MDA-MB-468 Cell SK-BR-3 Cell 293 Cell 293T/17 Cell Recommended protocols for these cells will be provided with the reagent. The protocols usually provide satisfactory transfection efficiency with invisible cytotoxicity. However, optimization may be needed for certain type of cells. Optimizations may include: the amount of DNA and this transfection reagent; cell density; transfection reagent/DNA ratio, or incubation time for the mixture of transfection reagent/DNA etc. For best transfection result, we recommend using the respective cell type/cell line specific Avalanche transfection reagents. Those reagents have been optimized on both recipes and protocols, and have been proved to have the best transfection results for the respective cell lines or primary cells. You can easily find the respective Avalanche transfection reagents specific for your cells by using the filters of our product list. |
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