In Vitro Toxicology: Mutagenicity kits

The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay. The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.

Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures

• Easy, compact and high throughput liquid format with 384 well plates.

• Simple detection method with easy "readout" (as compared to the traditional assay)

• Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline

• Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)

• Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound

• Significantly less consumption of S9

• Less environmental pollution due to significantly less "contaminated" waste

• Less user intervention

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