In Vitro Toxicology: Mutagenicity kits
The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay. The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.
Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures
Easy, compact and high throughput liquid format with 384 well plates.
Simple detection method with easy "readout" (as compared to the traditional assay)
Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline
Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)
Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound
Significantly less consumption of S9
Less environmental pollution due to significantly less "contaminated" waste
Less user intervention
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umuC Easy AQ and CS Test Kits
The umuC assay is based on the SOS response in S. typhimurium TA1535 pSK1002. The SOS response is induced as a consequence to DNA damage or disruption of DNA replication For convenience, positive controls and Lyophilized rat liver S9 are included in the kit.Howeverthe end user needs to specify.please see descriptionfor details.
Xenometrix AG
Catalogue No. | Description | Pack Size | Price | Qty |
|
---|---|---|---|---|---|
PPC-AA01 | 2-Aminoanthracene - Positive Control for S9 | 1 vial | £0.00 | Quantity | Add to Order |
XPRS-AC01 | lyophilized rat liver S9, Aroclor 1254 induced | 1 ml | £124.00 | Quantity | Add to Order |
umuC Easy AQ and CS Test Kits
The umuC assay is based on the SOS response in S. typhimurium TA1535 pSK1002. The SOS response is induced as a consequence to DNA damage or disruption of DNA replication For convenience, positive controls and Lyophilized rat liver S9 are included in the kit.Howeverthe end user needs to specify.please see descriptionfor details.
Xenometrix AG
The umuC assay is based on the SOS response in S. typhimurium TA1535 pSK1002. The SOS response is induced as a consequence to DNA damage or disruption of DNA replication. The reporter fusion gene umuC-lacZ on the plasmid pSK1002 is expressed as a consequence of mutational events induced by chemicals or UV radiation. This test is rapid and responds to chemicals with various mutational mechanisms, including cross linking events. The Xenometrix umuC Easy assays are based on the ISO 13829 international standard protocol using the same media and reagents. We have, however, modified the procedure for easier handling and provide an Excel calculation template for all necessary calculations. Xenometrix offers 2 versions of the kit: umuC Easy AQ for testing of aqueous samples, and umuC Easy CS for testing of concentrated samples like chemicals or extracts. Both umuC Easy kits have sufficient bacteria and reagents to perform 2 x 96 determinations. This allows to test 2 x 3 samples according to ISO 13829 in 4 dilutions, triplicate values, positive and negative controls, with and without S9 metabolic activation.
Contents
6 sample kit - 192 measuring points
2 vials SemiSolid TA1535psK1002
4 vials Ampicillin
Media and Reagents
To complete your kit with positive controls, lyophilized rat liver S9, Aroclor 1254 induced Please add:
1 vial Lyophilized Rat Liver S9, Aroclor induced 1.0 ml | XPRS-AC01 (£0.00) |
1 vial 2-Aminoanthracene - Positive Control for S9 | XPPC-AA01 (£0.00) |
1 vial 4-Nitroquinoline-N-Oxide - Positive Control for TA1535psK1002 | XPPC-NQ02 (£0.00) |
All media and strains provided with the kit are sufficient to analyze at least 6 samples in triplicates at 4 concentrations/- S9, plus neg. and pos. controls
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200