In Vitro Toxicology: Mutagenicity kits
The Ames II / Ames MPF Microfluctuation Assays are all modifications of the traditional Ames plate incorporation assay. The Ames II assay uses the S. typhimurium strains TA98 and TAMix (a mixture of 6 strains, each detecting a specific base-pair substitution), whereas the Ames MPF® kits are available with the strains TA98, TA100, TA1535, TA1537, E.coli WP2 uvrA or E.coli WP2 pKM101.
Benefits of Xenometrix Ames Mutagenicity Assays over traditional Ames Procedures
Easy, compact and high throughput liquid format with 384 well plates.
Simple detection method with easy "readout" (as compared to the traditional assay)
Kits include all five types strains cited in OECD 471 (CoA supplied), quality controlled according to the same guideline
Much less test compound required. (10 mg per strain if tested in triplicates, +/- S9, 6 semi-logarithmic compound dilutions)
Ames MPF is the preferred system for genotoxic impurities due to its low consumption of compound
Significantly less consumption of S9
Less environmental pollution due to significantly less "contaminated" waste
Less user intervention
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Ames MPF Penta 2kits
Ames MPF PENTA 2 is identical to Ames MPF PENTA 1, but it includes the strain E.coli uvrA[pkM101] instead of the two strains E.coli WP2 uvrA and E.coli WP2 [pkM101] resulting in the E.coli Combo.
Xenometrix AG
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| XB01-513-S1-P | Ames MPF Penta 2 - 1 sample 48 measuring points with S9 (aroclor) and Pos Control | 1 sample | £1,112.00 | Quantity | Add to Order |
| XB01-513-S2-P | Ames MPF Penta 2 - 1 sample 48 measuring points with S9 (Phenobarbital) and Pos Control | 1 sample | £1,112.00 | Quantity | Add to Order |
| XB10-513 | Ames MPF Penta 2 -10 sample (480) measuring points | 10 sample | £3,425.00 | Quantity | Add to Order |
| XB10-513-S1-P | Ames MPF Penta 2 -10 sample with S9 (aroclor) and Pos controls | 10 samples | £4,141.00 | Quantity | Add to Order |
| XB10-513-S2-P | Ames MPF Penta 2 10 sample with S9 (Phenobarbital) and Pos controls | 10 samples | £4,105.00 | Quantity | Add to Order |
Related products
Ames MPF Penta 2kits
Ames MPF PENTA 2 is identical to Ames MPF PENTA 1, but it includes the strain E.coli uvrA[pkM101] instead of the two strains E.coli WP2 uvrA and E.coli WP2 [pkM101] resulting in the E.coli Combo.
Xenometrix AG
New kit Ames MPF PENTA 2 is identical to Ames MPF PENTA 1, but it includes the straininstead of the two strains E.coli WP2 uvrA and E.coli WP2 [pkM101] resulting in the E.coli Combo. The new kit has the advantage of having only 1 E.coli strain which is listed in OECD 471. Proficiency chemicals have been tested.
For details of the Ames Penta technology and how it works please click here
Ames MPF Penta 1 (Short Protocol)
Assay principle
Bacteria are exposed to 6 concentrations of a test agent, as well as a positive and a negative control, for 90 minutes in medium containing sufficient histidine (S. typhimurium) or tryptophan (E.coli) to support approximately two cell divisions. After exposure, the cultures are diluted in pH indicator medium lacking histidine or tryptophan and aliquoted into 48 wells of a 384-well plate. Within two days, cells that have undergone reversion to amino acid prototrophy will grow into colonies.
Bacterial metabolism reduces the pH of the medium, changing the color of that well. The number of wells containing revertant colonies are counted for each dose and compared to a solvent (negative)control. Each dose is done in triplicate to allow for statistical analysis of the data. A dose dependent increase in the number of revertant colonies upon exposure to test sample relative to the solvent control indicates that the sample is mutagenic in the Ames MPF assay.The mutagenic potential of samples is assessed directly and in the presence of liver S9 fractions.
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If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
Ames MPF Penta 2kits
Ames MPF PENTA 2 is identical to Ames MPF PENTA 1, but it includes the strain E.coli uvrA[pkM101] instead of the two strains E.coli WP2 uvrA and E.coli WP2 [pkM101] resulting in the E.coli Combo.
Xenometrix AG
Click here for flyer
If you cannot find the answer to your problem below then please contact us or telephone 01954 210 200
