Oligo Synthesis : CEPs
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Spacer Phosphoramidite 18
Spacer Phosphoramidite 18
Glen Research
| Catalogue No. | Description | Pack Size | Price | Qty |
|
|---|---|---|---|---|---|
| 10-1918-02 | Spacer Phosphoramidite 18 | 0.25g | £192.00£182.40Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
| 10-1918-90 | Spacer Phosphoramidite 18 | 100µmoles | £76.00£72.20Offer until : 31-Mar-2021Offer Code : GLEN5View Offer | Quantity | Add to Order |
Spacer Phosphoramidite 18
Spacer Phosphoramidite 18
Glen Research
| Spacer Phosphoramidite 18 |
Catalog Number: 10-1918-xx
Description: Spacer Phosphoramidite 18
| 18-O-Dimethoxytritylhexaethyleneglycol,1-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite | ||
| Formula: C42H61N2O10P | M.W.: 784.93 | F.W.: 344.30 |
Diluent: Anhydrous AcetonitrileAdd fresh diluent to product vial to recommended concentration and swirl vial occasionally over several minutes until product is completely dissolved. (Some oils may require between 5 and 10 minutes.) Use care to maintain anhydrous conditions. In case of transfer to alternate vial type, ensure recipient vial has been pre-dried. For more information, seehttp://www.glenres.com/Technical/TB_ABITransfer.pdf. |
| Coupling: No changes needed from standard method recommended by synthesizer manufacturer. |
| Deprotection: No changes needed from standard method recommended by synthesizer manufacturer. |
| Storage: Freezer storage, -10 to -30°C, dry |
| Stability in Solution: 2-3 days |
SPACER MODIFIERS
The spacer phosphoramidites C3, 9, C12 and 18 are used to insert a spacer arm in an oligonucleotide. The compounds may be added in multiple additions when a longer spacer is required. 3’-Spacer C3 CPG may also act as a blocker of exonuclease and polymerase activity at the 3’-terminus. dSpacer is used to introduce a stable abasic site within an oligonucleotide. PC Spacer is a photocleavable C3 spacer modifier, part of our line of photocleavable (PC) modifiers
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Spacer Phosphoramidite 18
Spacer Phosphoramidite 18
Glen Research
Material Safety Data Sheet
Glen Report 7.1: NEW MODIFICATION REAGENTS AND SUPPORTS
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Spacer Phosphoramidite 18
Spacer Phosphoramidite 18
Glen Research
DILUTION/COUPLING DATA
The table below shows pack size data and, for solutions, dilutions and approximate couplings based on normal priming procedures. Please link formore detailed usage informationwith the various synthesizers.
| ABI 392/394 | |||||||||
| Cat.No. | PackSize | Grams/Pack | 0.1M Dil.(mL) | LV40 | LV200 | 40nm | 0.2µm | 1µm | 10µm |
| Approximate Number of Additions | |||||||||
| 10-1918-02 | 0.25grams | .25grams | 3.18 | 92.67 | 55.6 | 34.75 | 25.27 | 18.53 | 4.63 |
| 10-1918-90 | 100µmoles | .078grams | 1 | 20 | 12 | 7.5 | 5.45 | 4 | 1 |
| Expedite | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 50nm | 0.2µm | 1µm | 15µm | |
| Approximate Number of Additions | |||||||||
| 10-1918-02 | 0.25grams | .25grams | 4.75 | .07 | 88.6 | 55.38 | 40.27 | 5.54 | |
| 10-1918-90 | 100µmoles | .078grams | 1.5 | .07 | 23.6 | 14.75 | 10.73 | 1.48 | |
| Beckman | |||||||||
| Cat.No. | PackSize | Grams/Pack | Dilution(mL) | Molarity | 30nm | 200nm | 1000nm | ||
| Approximate Number of Additions | |||||||||
| 10-1918-02 | 0.25grams | .25grams | 4.75 | .07 | 90.2 | 56.38 | 41 | ||
| 10-1918-90 | 100µmoles | .078grams | 1.5 | .07 | 25.2 | 15.75 | 11.45 | ||
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